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<?xml version="1.0" encoding="UTF-8"?>
<doi_batch version="1.0.0">
<!-->1.头标区<-->
<head>
<doi_batch_id>20111020</doi_batch_id>
<timestamp>20111020144942</timestamp>
<depositor>
<name>wangyue</name>
<email_address>wangyue81@gmail.com</email_address>
</depositor>
<registrant>zgfazz</registrant>
</head>
<!-->2.主体<-->
<body>
<!-->中国肺癌杂志<-->
<journal>
<!-->2.1 刊元数据<-->
<journal_metadata>
<journal_id>zgfazz</journal_id>
<full_title language="zh">中国肺癌杂志</full_title>
<full_title language="en">Chinese Journal of Lung Cancer</full_title>
<abbrev_title/>
<issn media_type="print">1009-3419</issn>
<cn media_type="print">12-1395/R</cn>
</journal_metadata>
<!-->2.2 期元数据<-->
<journal_issue>
<publication_date media_type="print">
<!--需media_type: online | print | other-->
<year>2011</year>
<month/>
<day/>
</publication_date>
<journal_volume>
<volume>14</volume>
</journal_volume>
<issue>10</issue>
<special_numbering/>
</journal_issue>
<!-->2.3 文章元数据<-->
<journal_article>
<titles language="zh">
<title>高分辨熔解曲线法检测非小细胞肺癌p53基因的突变</title>
<!-->一个titles里面只能有一个title<-->
<subtitle/>
</titles>
<titles language="en">
<title>High Resolution Melting Analysis for Detecting p53 Gene Mutations in Patients with Non-small Cell Lung Cancer</title>
<subtitle/>
</titles>
<contributors>
<person_name language="zh" sequence="first" contributor_role="author">陈志红</person_name>
<person_name language="zh" sequence="additional" contributor_role="author">安社娟</person_name>
<person_name language="zh" sequence="additional" contributor_role="author">谢至</person_name>
<person_name language="zh" sequence="additional" contributor_role="author">严红虹</person_name>
<person_name language="zh" sequence="additional" contributor_role="author">陈剑光</person_name>
<person_name language="zh" sequence="additional" contributor_role="author">苏健</person_name>
<person_name language="zh" sequence="additional" contributor_role="author">张绪超</person_name>
<person_name language="zh" sequence="additional" contributor_role="author">牛飞玉</person_name>
<person_name language="zh" sequence="additional" contributor_role="author">郭伟浜</person_name>
<person_name language="zh" sequence="additional" contributor_role="author">吴一龙</person_name>
<person_name language="en" sequence="first" contributor_role="author">Zhihong CHEN</person_name>
<person_name language="en" sequence="additional" contributor_role="author">Shejuan AN</person_name>
<person_name language="en" sequence="additional" contributor_role="author">Zhi XIE</person_name>
<person_name language="en" sequence="additional" contributor_role="author">Honghong YAN</person_name>
<person_name language="en" sequence="additional" contributor_role="author">Jianguang CHEN</person_name>
<person_name language="en" sequence="additional" contributor_role="author">Jian SU</person_name>
<person_name language="en" sequence="additional" contributor_role="author">Xuchao ZHANG</person_name>
<person_name language="en" sequence="additional" contributor_role="author">Feiyu NIU</person_name>
<person_name language="en" sequence="additional" contributor_role="author">Weibang GUO</person_name>
<person_name language="en" sequence="additional" contributor_role="author">Yilong WU</person_name>
<organization sequence="first" contributor_role="author">510080 广州，广东省人民医院医学研究中心，广东省医学科学院，广东省肺癌研究所（ 通讯作者：吴一龙，E-mail: syylwu@live.cn）;Medical Research Center, Guangdong General Hospital, Guangdong Lung Cancer Institute, 
Guangdong Academy of Medical Sciences, Guangzhou 510080, China</organization>
</contributors>
<publication_date media_type="print">
<year>2011</year>
</publication_date>
<publisher_item>
<item_number>zgfazz201110005</item_number>
</publisher_item>
<keywords language="zh">p53基因；突变；测序</keywords>
<keywords language="en">p53 gene; Mutation; Sequence</keywords>
<abstract language="zh">背景与目的 p53基因与人类多种肿瘤相关，突变型具有致癌作用，主要分布在外显子5-8。本研究旨在建立高分辨熔解曲线（high resolution melting, HRM）检测非小细胞肺癌（non-small cell lung cancer, NSCLC）患者p53基因突变的方法，探讨p53基因突变的特点及其在NSCLC发生发展中的演变规律。方法 采用HRM法检测264例NSCLC患者肿瘤组织和54例癌旁肺组织p53基因外显子5-8的突变，突变样品进一步使用PCR产物直接测序法分析确定突变类型；HRM法检测阳性而PCR产物直接测序法检测阴性的样品，进一步进行亚克隆测序证实。结果 54例对照未发现突变。264例肿瘤组织中，HRM法检出 p53基因突变104例，102例得到PCR产物直接测序法证实，突变率为39.4%；95例为点突变，7例为碱基插入和缺失导致的移码突变。p53外显子5-8的突变率分别为11.7%、8%、12.5%和10.6%，差异无统计学意义（P=0.35）。p53基因突变与性别有关，与其它临床病理特征无关。结论 HRM法筛选p53基因突变样品，具有操作简便、快速、敏感、单管避免污染等优点，值得推广。p53基因的突变特点提示，p53基因突变是自发性突变，可能是DNA合成和修复过程中的随机错误所致。</abstract>
<abstract language="en">Background and objective It has been proven that p53 gene was related to many human cancers. The mutations in p53 gene play an important role in carcinogensis and mostly happened in exon 5-8. The aim of this study is to establish a high resolution melting (HRM) assay to detect p53 mutations from patients with non-small cell lung cancer (NSCLC), to investigate the characteristics of p53 gene mutations, and to analyze the relationship between p53 mutations and evolution regularity of pathogenesis. Methods p53 mutations in exon 5-8 were detected by HRM assay on DNA insolated from 264 NSCLC samples derived from tumor tissues and 54 control samples from pericancerous pulmonary tissues. The mutation samples by the HRM assay were confirmed by sequencing technique. Samples which were positive by HRM but wild type by sequencing were further confirmed by sub-clone and sequencing. Results No mutation was found in 54 pericancerous pulmonary samples by HRM assay. 104 of the 264 tumor tissues demonstrated mutation curves by HRM assay, 102 samples were confirmed by sequencing, including 95 point mutations and 7 frame shift mutations by insertion or deletion. The mutation rate of p53 gene was 39.4%. The mutation rate from exon 5-8 were 11.7%, 8%, 12.5% and 10.6%, respectively and there was no statistically significant difference between them (P=0.35). p53 mutations were significantly more frequent in males than that in females, but not related to the other clinicopathologic characteristics. Conclusion The results indicate that HRM is a sensitive in-tube methodology to detect for mutations in clinical samples. The results suggest that the arising p53 mutations in NSCLC may be due to spontaneous error in DNA synthesis and repair.</abstract>
<doi_data>
<doi>10.3779/j.issn.1009-3419.2011.10.01</doi>
<resource><![CDATA[http://www.lungca.org/index.php?journal=01&page=article&op=view&path[]=10.3779%2Fj.issn.1009-3419.2011.10.01&path[]=html]]></resource>
<collection property="list-based" multi-resolution="unlock">
<item label="XXX中文版" country="CN" >
<resource><![CDATA[http://www.xxxx.com/cn]]></resource>
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<item label="XXX英文版" country="CN" >
<resource><![CDATA[http://www.xxxx.com/en]]></resource>
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<pages>
<first_page>767</first_page>
<last_page>773</last_page>
</pages>
<citation_list>
<citation language="en">
<citation_key>1</citation_key>
<author>Levesque AA,Eastman A</author>
<title>p53-based cancer therapies:Is defective p53 the Achilles heel of the tumor</title>
<source_type>J</source_type>
<source_title>Carcinogenesis</source_title>
<cYear>2007</cYear>
<issue>01</issue>
<first_page>13</first_page>
<last_page>20</last_page>
<doi>10.1093/carcin.bgl214</doi>
</citation>
<citation language="en">
<citation_key>2</citation_key>
<author>Olivier M,Hollstein M,Hainaut P</author>
<title>TP53 mutations in human cancers:origins,consequences,and clinical use</title>
<source_type>J</source_type>
<source_title>Cold Spring Harbor Perspectives in Biology</source_title>
<cYear>2010</cYear>
<issue>01</issue>
<doi>10.1101/cshperspect.a001008</doi>
</citation>
<citation language="zh">
<citation_key>3</citation_key>
<author>陈志红,郭爱林,安社娟</author>
<title>HRM法检测结直肠癌组织KRAS基因密码子12和13点突变</title>
<source_type>J</source_type>
<source_title>中华检验医学杂志</source_title>
<cYear>2010</cYear>
<issue>03</issue>
<first_page>209</first_page>
<last_page>212</last_page>
<doi>10.3760/cma.j.issn.1009-9158.2010.03.004</doi>
</citation>
<citation>
<citation_key>4</citation_key>
<title>TP53 mutations in Korean patients with nonsmall cell lung cancer</title>
<unstructured_citation>Lee EB,Jin G,Lee SY. TP53 mutations in Korean patients with nonsmall cell lung cancer[J].Journal of Korean Medical Science,2010,(05):698-705</unstructured_citation>
</citation>
</citation_list>
</journal_article>
</journal>
</body>
</doi_batch>

144
vendor/chinadoi/upload/test002.xml vendored Normal file
View File

@@ -0,0 +1,144 @@
<?xml version="1.0" encoding="UTF-8"?>
<doi_batch version="1.0.0">
<!-->1.头标区<-->
<head>
<doi_batch_id>20111020</doi_batch_id>
<timestamp>20111020144942</timestamp>
<depositor>
<name>wangyue</name>
<email_address>wangyue81@gmail.com</email_address>
</depositor>
<registrant>zgfazz</registrant>
</head>
<!-->2.主体<-->
<body>
<!-->中国肺癌杂志<-->
<journal>
<!-->2.1 刊元数据<-->
<journal_metadata>
<journal_id>zgfazz</journal_id>
<full_title language="zh">中国肺癌杂志</full_title>
<full_title language="en">Chinese Journal of Lung Cancer</full_title>
<abbrev_title/>
<issn media_type="print">1009-3419</issn>
<cn media_type="print">12-1395/R</cn>
</journal_metadata>
<!-->2.2 期元数据<-->
<journal_issue>
<publication_date media_type="print">
<!--需media_type: online | print | other-->
<year>2011</year>
<month/>
<day/>
</publication_date>
<journal_volume>
<volume>14</volume>
</journal_volume>
<issue>10</issue>
<special_numbering/>
</journal_issue>
<!-->2.3 文章元数据<-->
<journal_article>
<titles language="zh">
<title>高分辨熔解曲线法检测非小细胞肺癌p53基因的突变</title>
<!-->一个titles里面只能有一个title<-->
<subtitle/>
</titles>
<titles language="en">
<title>High Resolution Melting Analysis for Detecting p53 Gene Mutations in Patients with Non-small Cell Lung Cancer</title>
<subtitle/>
</titles>
<contributors>
<person_name language="zh" sequence="first" contributor_role="author">陈志红</person_name>
<person_name language="zh" sequence="additional" contributor_role="author">安社娟</person_name>
<person_name language="zh" sequence="additional" contributor_role="author">谢至</person_name>
<person_name language="zh" sequence="additional" contributor_role="author">严红虹</person_name>
<person_name language="zh" sequence="additional" contributor_role="author">陈剑光</person_name>
<person_name language="zh" sequence="additional" contributor_role="author">苏健</person_name>
<person_name language="zh" sequence="additional" contributor_role="author">张绪超</person_name>
<person_name language="zh" sequence="additional" contributor_role="author">牛飞玉</person_name>
<person_name language="zh" sequence="additional" contributor_role="author">郭伟浜</person_name>
<person_name language="zh" sequence="additional" contributor_role="author">吴一龙</person_name>
<person_name language="en" sequence="first" contributor_role="author">Zhihong CHEN</person_name>
<person_name language="en" sequence="additional" contributor_role="author">Shejuan AN</person_name>
<person_name language="en" sequence="additional" contributor_role="author">Zhi XIE</person_name>
<person_name language="en" sequence="additional" contributor_role="author">Honghong YAN</person_name>
<person_name language="en" sequence="additional" contributor_role="author">Jianguang CHEN</person_name>
<person_name language="en" sequence="additional" contributor_role="author">Jian SU</person_name>
<person_name language="en" sequence="additional" contributor_role="author">Xuchao ZHANG</person_name>
<person_name language="en" sequence="additional" contributor_role="author">Feiyu NIU</person_name>
<person_name language="en" sequence="additional" contributor_role="author">Weibang GUO</person_name>
<person_name language="en" sequence="additional" contributor_role="author">Yilong WU</person_name>
<organization sequence="first" contributor_role="author">510080 广州，广东省人民医院医学研究中心，广东省医学科学院，广东省肺癌研究所（ 通讯作者：吴一龙，E-mail: syylwu@live.cn）;Medical Research Center, Guangdong General Hospital, Guangdong Lung Cancer Institute, 
Guangdong Academy of Medical Sciences, Guangzhou 510080, China</organization>
</contributors>
<publication_date media_type="print">
<year>2011</year>
</publication_date>
<publisher_item>
<item_number>zgfazz201110005</item_number>
</publisher_item>
<keywords language="zh">p53基因；突变；测序</keywords>
<keywords language="en">p53 gene; Mutation; Sequence</keywords>
<abstract language="zh">背景与目的 p53基因与人类多种肿瘤相关，突变型具有致癌作用，主要分布在外显子5-8。本研究旨在建立高分辨熔解曲线（high resolution melting, HRM）检测非小细胞肺癌（non-small cell lung cancer, NSCLC）患者p53基因突变的方法，探讨p53基因突变的特点及其在NSCLC发生发展中的演变规律。方法 采用HRM法检测264例NSCLC患者肿瘤组织和54例癌旁肺组织p53基因外显子5-8的突变，突变样品进一步使用PCR产物直接测序法分析确定突变类型；HRM法检测阳性而PCR产物直接测序法检测阴性的样品，进一步进行亚克隆测序证实。结果 54例对照未发现突变。264例肿瘤组织中，HRM法检出 p53基因突变104例，102例得到PCR产物直接测序法证实，突变率为39.4%；95例为点突变，7例为碱基插入和缺失导致的移码突变。p53外显子5-8的突变率分别为11.7%、8%、12.5%和10.6%，差异无统计学意义（P=0.35）。p53基因突变与性别有关，与其它临床病理特征无关。结论 HRM法筛选p53基因突变样品，具有操作简便、快速、敏感、单管避免污染等优点，值得推广。p53基因的突变特点提示，p53基因突变是自发性突变，可能是DNA合成和修复过程中的随机错误所致。</abstract>
<abstract language="en">Background and objective It has been proven that p53 gene was related to many human cancers. The mutations in p53 gene play an important role in carcinogensis and mostly happened in exon 5-8. The aim of this study is to establish a high resolution melting (HRM) assay to detect p53 mutations from patients with non-small cell lung cancer (NSCLC), to investigate the characteristics of p53 gene mutations, and to analyze the relationship between p53 mutations and evolution regularity of pathogenesis. Methods p53 mutations in exon 5-8 were detected by HRM assay on DNA insolated from 264 NSCLC samples derived from tumor tissues and 54 control samples from pericancerous pulmonary tissues. The mutation samples by the HRM assay were confirmed by sequencing technique. Samples which were positive by HRM but wild type by sequencing were further confirmed by sub-clone and sequencing. Results No mutation was found in 54 pericancerous pulmonary samples by HRM assay. 104 of the 264 tumor tissues demonstrated mutation curves by HRM assay, 102 samples were confirmed by sequencing, including 95 point mutations and 7 frame shift mutations by insertion or deletion. The mutation rate of p53 gene was 39.4%. The mutation rate from exon 5-8 were 11.7%, 8%, 12.5% and 10.6%, respectively and there was no statistically significant difference between them (P=0.35). p53 mutations were significantly more frequent in males than that in females, but not related to the other clinicopathologic characteristics. Conclusion The results indicate that HRM is a sensitive in-tube methodology to detect for mutations in clinical samples. The results suggest that the arising p53 mutations in NSCLC may be due to spontaneous error in DNA synthesis and repair.</abstract>
<doi_data>
<doi>10.3779/j.issn.1009-3419.2011.10.01</doi>
<resource><![CDATA[http://www.lungca.org/index.php?journal=01&page=article&op=view&path[]=10.3779%2Fj.issn.1009-3419.2011.10.01&path[]=html]]></resource>
<collection property="list-based" multi-resolution="unlock">
<item label="XXX中文版" country="CN" >
<resource><![CDATA[http://www.xxxx.com/cn]]></resource>
</item>
<item label="XXX英文版" country="CN" >
<resource><![CDATA[http://www.xxxx.com/en]]></resource>
</item>
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</doi_data>
<pages>
<first_page>767</first_page>
<last_page>773</last_page>
</pages>
<citation_list>
<citation language="en">
<citation_key>1</citation_key>
<author>Levesque AA,Eastman A</author>
<title>p53-based cancer therapies:Is defective p53 the Achilles heel of the tumor</title>
<source_type>J</source_type>
<source_title>Carcinogenesis</source_title>
<cYear>2007</cYear>
<issue>01</issue>
<first_page>13</first_page>
<last_page>20</last_page>
<doi>10.1093/carcin.bgl214</doi>
</citation>
<citation language="en">
<citation_key>2</citation_key>
<author>Olivier M,Hollstein M,Hainaut P</author>
<title>TP53 mutations in human cancers:origins,consequences,and clinical use</title>
<source_type>J</source_type>
<source_title>Cold Spring Harbor Perspectives in Biology</source_title>
<cYear>2010</cYear>
<issue>01</issue>
<doi>10.1101/cshperspect.a001008</doi>
</citation>
<citation language="zh">
<citation_key>3</citation_key>
<author>陈志红,郭爱林,安社娟</author>
<title>HRM法检测结直肠癌组织KRAS基因密码子12和13点突变</title>
<source_type>J</source_type>
<source_title>中华检验医学杂志</source_title>
<cYear>2010</cYear>
<issue>03</issue>
<first_page>209</first_page>
<last_page>212</last_page>
<doi>10.3760/cma.j.issn.1009-9158.2010.03.004</doi>
</citation>
<citation>
<citation_key>4</citation_key>
<title>TP53 mutations in Korean patients with nonsmall cell lung cancer</title>
<unstructured_citation>Lee EB,Jin G,Lee SY. TP53 mutations in Korean patients with nonsmall cell lung cancer[J].Journal of Korean Medical Science,2010,(05):698-705</unstructured_citation>
</citation>
</citation_list>
</journal_article>
</journal>
</body>
</doi_batch>